Effect of sulfamethoxazole on biological phosphorus removal and its mechanism

doi:10.19965/j.cnki.iwt.2023-0033

Abstract

Abstract:

As a typical representative of sulfonamides，sulfamethoxazole （SMZ） has a potential threat to biological phosphorus removal process. In order to explore its impact on biological phosphorus removal performance and its mechanism，a sequencing batch reactor was constructed to determine the impact of sulfamethoxazole on reactor performance，explore the impact of sulfamethoxazole on the content of microbial metabolic intermediates（poly-P，PHB and glycogen） and the activity of key enzymes（PPX，PPK）. Moreover， the micro mechanism of sulfamethoxazole on key enzymes were analyzed with molecular docking technology. The results showed that low concentrations of sulfamethoxazole（0.05 mg/L and 0.1 mg/L） had no significant effect on biological phosphorus removal performance，the content of metabolic intermediates and the activity of key enzymes. When the concentration of sulfamethoxazole increased to 1 mg/L，it began to inhibit biological phosphorus removal performance. With the increase of its concentration，the inhibition increased. Furthermore， the synthesis and degradation of poly-P and PHB in microbial cells decreased with the increase of the concentration of sulfamethoxazole， while the synthesis and degradation of glycogen will increase significantly. The inhibition rate of sulfamethoxazole on key enzyme activities increased with the rise of concentration. The molecular docking results showed that sulfamethoxazole combined with the amino acid residues in the enzyme activity center to form a complex，thus changing the structure of the enzyme.

Key words: sulfamethoxazole, biological phosphorus removal, sequencing batch reactor, molecular docking

摘要：

磺胺甲唑（SNZ）作为磺胺类药物的典型代表，会对生物除磷过程构成潜在威胁。为探究其对生物除磷性能的影响及其机制，构建了序批式反应器，测定磺胺甲唑对反应器性能的影响，探究磺胺甲唑对微生物代谢中间产物（poly-P、PHB及糖原）含量及关键酶（PPX、PPK）活性的影响，并利用分子对接技术分析磺胺甲唑对关键酶作用的微观机制。结果表明，低质量浓度的磺胺甲唑（0.05、0.1 mg/L）对生物除磷性能、代谢中间产物的含量及关键酶活性无显著影响，当磺胺甲唑质量浓度提升至1 mg/L时，开始对生物除磷性能表现出抑制作用，且随着浓度的升高，抑制作用增强，微生物胞内poly-P、PHB的合成及降解量会随着磺胺甲唑浓度的升高而降低，糖原的合成及降解量则会显著增加。磺胺甲唑对关键酶活性的抑制率也会随着浓度的升高而增加，分子对接结果表明磺胺甲唑会与酶活性中心的氨基酸残基结合形成复合物从而改变酶的结构。

关键词: 磺胺甲唑, 生物除磷, 序批式反应器, 分子对接

CLC Number:

X703

Haoyang XIN, Jiajun Li. Effect of sulfamethoxazole on biological phosphorus removal and its mechanism[J]. Industrial Water Treatment, 2024, 44(2): 79-86.

辛浩洋, 李家俊. 磺胺甲唑对生物除磷性能的影响及机制[J]. 工业水处理, 2024, 44(2): 79-86.

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URL: https://www.iwt.cn/EN/10.19965/j.cnki.iwt.2023-0033

https://www.iwt.cn/EN/Y2024/V44/I2/79

Figures/Tables 9

Fig.1 Reactor device diagram

Fig.2 Effect of sulfamethoxazole on COD（a） and SOP（b） contents in reactor effluent

Fig.3 Effect of sulfamethoxazole on SOP content in typical cycle

Fig.4 Effect of sulfamethoxazole on Poly-P content change in typical periods

Fig.5 Effect of sulfamethoxazole on the change of PHB content in typical cycle

Fig.6 Effect of sulfamethoxazole on the change of glycogen content in typical cycle

Fig.7 Relative inhibition rate of sulfamethoxazole on key enzymes

Fig.8 Molecules docking results of sulfamethoxazole with PPX and PPK

Fig.9 Mechanism diagram of sulfamethoxazole on microorganism in phosphorus removal process

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