摘要：

通过构建双水相萃取体系，从马铃薯淀粉加工废水中萃取过氧化物酶，再经透析、冻干得马铃薯过氧化物酶固体酶粉，用于催化H₂O₂快速降解双酚A(BPA)。结果表明，在室温，pH为6，200 U/mL马铃薯过氧化物酶催化1.0 mmol/L H₂O₂氧化初始浓度为0.8 mmol/L BPA的条件下，反应10 min后，BPA的降解率可达99%以上，对于修复水体具有极大的潜在应用价值。

关键词: 马铃薯, 过氧化物酶, 双酚A, 降解

Abstract:

A two-phase aqueous extraction system was established to extract peroxidase from potato starch wastewater, and then the enzyme was obtained by dialysis and freeze-drying. The obtained enzyme was used to catalyze the oxidation of bisphenol A(BPA) by H₂O₂. The results showed that 200 U/mL of potato peroxidase catalyzed the oxidation of 0.8 mmol/L bisphenol A with 1.0 mmol/L H₂O₂ at room temperature and pH 6. After 10 min, the degradation rate of bisphenol A was up to 99%. It shows great potential application value for repairing water body.

Key words: potato, peroxidase, bisphenol A, degradation

中图分类号: 

• X703.1