摘要：

以厌氧氨氧化菌（AAOB）驱动的Anammox反应为主流城镇污水处理厂（WWTPs）的发展提供了新思路，但AAOB的多样性在主流WWTPs中尚不清楚。利用高通量测序技术探析了4个主流WWTPs处理工艺的不同处理单元中AAOB的多样性。结果表明，主流WWTPs中A²/O、氧化沟和间歇进水循环式活性污泥工艺（CAST）的各生物单元均存在AAOB，但多样性较低，相同WWTPs不同生物单元的AAOB组成相似性较高。Planctomycetes门丰度在41.873%~96.565%，AAOB由CandidatusBrocadia、CandidatusKuenenia、Candidatus Anammoxoglobus、unclassified Candidatus Brocadiaceae和未明确分类地位的菌属组成，优势AAOB为unclassified Candidatus Brocadiaceae，丰度在33.363%~95.346%。研究结果可为主流Anammox工艺城镇污水处理发展提供理论基础。

关键词: 城镇污水处理厂, 厌氧氨氧化菌, 多样性, 高通量测序

Abstract:

Anammox reaction driven by anaerobic ammonium oxidizing bacteria(AAOB) provides a new idea for the development of mainstream municipal wastewater treatment plants(WWTPs). The diversity of AAOB community is not yet clear in mainstream WWTPs. High-throughput sequencing technology was used to explore AAOB diversity in different processing units in four mainstream WWTPs with different processes. The results showed that AAOB existed in each biological unit of A²/O, oxidation ditch and CAST processes, but the diversity was low. Different biological units of the same WWTPs were similar in AAOB composition. The abundance of Planctomycetes ranged from 41.873% to 96.565%, and AAOB consisted of Candidatus Brocadia, Candidatus Kuenenia, Candidatus Anammoxoglobus, unclassified Candidatus Brocadiaceae and unclassified genera. The dominant AAOB was unclassified Candidatus Brocadiaceae with abundance ranging from 33.363% to 95.346%. The results could provide theoretical basis for the development of mainstream Anammox process for municipal wastewater treatment.

Key words: municipal wastewater treatment plant, anaerobic ammonium-oxidizing bacteria, diversity, high-through-put sequencing

中图分类号: 

• X703