红球菌KDPy1在焦化废水生物处理中的强化作用

doi:10.19965/j.cnki.iwt.2021-0059

摘要/Abstract

摘要：

喹啉、吡啶等含氮杂环化合物是焦化废水中主要的难降解有机物。以喹啉、吡啶作为目标污染物，研究了筛选出的高效降解菌红球菌（Rhodococcus sp.）KDPy1对焦化废水A/O²生物处理工艺的强化作用。结果表明，与对照组相比，红球菌的添加使O1池的COD、喹啉、吡啶去除率分别增加了11.4%、17.3%、14.0%。经生物强化后，系统内微生物群落多样性增加，且有机污染物降解菌如Stenotrophomonas和Ochrobactrum等更具优势，证实了红球菌（Rhodococcus sp.）KDPy1在焦化废水处理中的巨大应用前景。

关键词: 焦化废水, 生物强化, 含氮杂环化合物, 红球菌

Abstract:

Heterocyclic compounds containing nitrogen such as quinolone and pyridine, are the main refractory organic compounds in coking wastewater. In this study, taking pyridine and quinoline as object pollutants, the isolated strain Rhodococcus sp. KDPy1 was used to strengthen the A/O² process for coking wastewater treatment. The results showed that compared with the control group, the addition of Rhodococcus sp. increased the removal rate of COD, quinoline and pyridine in the O1 pool by 11.4%, 17.3% and 14.0%, respectively. At the same time, the diversity of microbial communities increased after bioaugmentation, and the organic degrading bacteria such as Stenotrophomonas and Ochrobactrum were more abundant in the system, which proved that Rhodococcus sp. KDPy1 had a great application potential in coking wastewater treatment.

Key words: coking wastewater, bioaugmentation, nitrogen heterocyclic compounds, Rhodococcus sp.

中图分类号:

X703

李婷,张玉秀,祖德彪,徐伟超. 红球菌KDPy1在焦化废水生物处理中的强化作用[J]. 工业水处理, 2021, 41(10): 96-103.

Ting LI,Yuxiu ZHANG,Debiao ZU,Weichao XU. Bioaugmentation of Rhodococcus sp. KDPy1 in coking wastewater treatment[J]. Industrial Water Treatment, 2021, 41(10): 96-103.

https://www.iwt.cn/CN/Y2021/V41/I10/96

图/表 8

图1 某焦化废水处理厂工艺流程

Fig.1 Process flow of a coking wastewater treatment plant

图2 A/O²反应器工艺流程
1—调节池；2—进水蠕动泵；3—缺氧池；4—搅拌器；5—沉淀池；6—污泥回流蠕动泵；7—好氧池1；8—曝气泵；9—曝气盘；10—好氧池2；11—硝化液回流蠕动泵。

Fig.2 A/O² reactor process diagram

图3 对照组（a）、实验组（b）以及好氧池O1（c）中COD的变化比较

Fig.3 Comparison of COD changes in control group(a), experimental group(b)and primary aerobic pool O1(c)

图4 A/O²系统各处理单元对氮的去除性能

Fig.4 Nitrogen removal performance of each treatment unit in A/O² system

图5 A/O²系统各处理单元对吡啶（a）和喹啉（b）的去除性能

Fig.5 pyridine(a)and quinolone(b) removal performance of each treatment unit in A/O² system

表1 进出水中有机物的归一化分析

Table 1 Normalized analysis of organic matter in inlet and outlet water

有机物种类	峰面积/%
有机物种类	进水	对照组出水（1-O2）	实验组出水（2-O2）
酚类	15.57	ND	ND
苯	0.21	3.25	1.75
烷烃	26.32	55.21	59.34
杂环	25.35	16.75	8.76
多环	0.72	ND	ND
羧酸	0.90	12.23	13.22
酮	0.17	ND	ND
醇类	29.47	0.88	1.91
腈类	0.06	ND	ND

表2 微生物群落OTUs和多样性参数

Table 2 Microbial community OTUs and diversity parameters

样本	反应池	OTUs	Shannon	ACE	Chao1	Coverage
对照组	A	1762	3.85	3875.33	2898.54	0.9896
	O1	1538	4.73	3324.85	2572.71	0.9904
	O2	1644	4.74	2870.43	2346.54	0.9926
实验组	A	1783	4.17	4095.89	2853.74	0.9897
	O1	1697	4.83	3592.44	2665.63	0.9914
	O2	1704	4.74	2630.35	2613.10	0.9920

图6 两系统内各单元在门水平（a）和属水平（b）上的菌群分布

Fig.6 Flora distribution of each unit in the two systems at phylum level(a)and genus level(b)

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